Cytokine Response of the Biomimetic Porcine Urothelial Model to Different Escherichia coli Strains

Escherichia coli is known to be an important uropathogenic agent. Several models were developed for investigating the uropathogensis of E. coli, including recent biomimetic porcine urothelial in vitro model. The aim this study was assess cytokine response cells model different strains. production nine cytokines infection evaluated using commercial pre-configured immunoassay multiplex Cytokine & Chemokine 9-Plex Porcine ProcartaPlex™ Panel 1 kit. Our results showed that reacted presence all employed strains, albeit with some differences levels and types produced. Increased IL-10, IL-8, TNF-α, IL-1β, IL-4 IL-12p40 observed. Statistical analysis (Fisher’s exact test) revealed a correlation between high fold change immune cnf1 gene encodes cytotoxic necrotizing factor. shed light on normal strains have potential fuel search understanding mechanisms behind responses